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KMID : 0377619730250010033
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Korean Jungang Medical Journal
1973 Volume.25 No. 1 p.33 ~ p.36
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Cell Proliferation Studies in Duodenal Crypt of the Chick
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Abstract
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Tritiated thymidine autoradiography has been applied to the duodenal progenitor population (crypts of Lieberkuhn) in one day postnatal male chick to determine 1) the cell cycle of the progenitor cell pool, 2) the characteristics of DNA synthetic index, and the 3) mitotic index.
Available amounts of colchicine were injected into the animals before sacrifice to arrest the dividing cell nucleus at metaphase.
The results of this investigation are as follows:
1) The generation time was 14 hours,
2) DNA synthetic index was 23.3%, and
3) The mitotic index was 1.8%.
The cause of shortening of DNA synthetic phase and the lengthening of post mitotic phase were discussed.
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KEYWORD
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